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A9 小鼠皮下结缔组织细胞

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产品名称: A9 小鼠皮下结缔组织细胞
产品型号: CCL-1.4
产品厂商: 美国标准生物品收藏中心(ATCC)
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A9 小鼠皮下结缔组织细胞ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞。细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件


A9 小鼠皮下结缔组织细胞 的详细介绍

A9 小鼠皮下结缔组织细胞

ATCC® Number:  CCL-1.4™      
Designations:  A9 (APRT and HPRT negative derivative of Strain L) 
Depositors:   TR Chen 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Mus musculus (mouse) 
Morphology: fibroblast

 A9 小鼠皮下结缔组织细胞
Source: Strain: C3H/An
Tissue: subcutaneous connective tissue; areolar and adipose
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Tumorigenic: Yes 
Antigen Expression: H-2k
Cytogenetic Analysis: modal number = 52; range = 46 to 54; This is a hyperdiploid cell line with 24 to 26 marker chromosomes common to all cells., Nine markers (M1 - M9) are common to L-M(TK-) (see ATCC CCL-1.3) and 16 to 17 are found only in A9. The latter markers include Rb(3.5), Rb(4.15), Rb(5.5), T(8;19), Rb(9.17) and 11 to 12 others., Structurally normal chromosomes 4, 15, 16, 17, 18 and X were absent.
Age:  100 days 
Gender:  male 
Comments: The A9 cell line was isolated by J.W. Littlefield from wild-type Strain L cells.
The cells are sensitive to HAT selection medium and deficient in adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT).
The cells are resistant to 8-azaguanine (0.003 mg/ml) and 6-thioguanine (0.1 mM).
Propagation:  ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5g/L glucose, 90%; fetal bovine serum, 10%
 A9 小鼠皮下结缔组织细胞
Subculturing:  Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove old medium, add fresh 0.25% trypsin, EDTA (0.03%) solution, rinse and remove. Allow cells to sit at room temperature (or at 37C) until cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products: parental cell line:ATCC CCL-1
References: 21409: Littlefield JW. The inosinic acid pyrophosphorylase activity of mouse fibroblasts partially resistant to 8-azaguanine. Proc. Natl. Acad. Sci. USA 50: 568-573, 1963. PubMed: 14067106
21410: Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673
27669: Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365 
 A9 小鼠皮下结缔组织细胞
 

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