产品[
Hs 738.St/Int 人成纤维细胞
]资料
如果您对该产品感兴趣的话,可以
产品名称:
Hs 738.St/Int 人成纤维细胞
产品型号:
CRL-7869
产品厂商:
美国标准生物品收藏中心(ATCC)
产品文档:
无相关文档
简单介绍
CRL-7869 Hs 738.St/Int 人成纤维细胞,原代细胞|细胞系|细胞株|肿瘤细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件!
Hs 738.St/Int 人成纤维细胞
的详细介绍
CRL-7869 Hs 738.St/Int 人成纤维细胞
|
CRL-7869™
|
|
|
|
Hs 738.St/Int
|
Biosafety Level:
|
1
|
|
frozen
|
Medium & Serum:
|
See Propagation
|
|
adherent
|
|
Homo sapiens (human)CRL-7869 Hs 738.St/Int 人成纤维细胞
|
|
fibroblast
|
|
Organ: mixed; stomach; intestine
Disease: normal
|
|
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
|
|
|
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,11
D16S539: 9,11
D5S818: 11,13
D7S820: 8,12
THO1: 7,9.3
TPOX: 8
vWA: 16,18
|
|
modal number = 46; range = 44 to 92
|
|
18 weeks gestation fetus
|
|
CRL-7869 Hs 738.St/Int 人成纤维细胞male
|
|
Caucasian
|
|
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
|
|
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
|
|
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor termperature
|
|
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
|
CRL-7869 Hs 738.St/Int 人成纤维细胞
|