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CRL-7869 Hs 738.St/Int 人成纤维细胞

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产品名称: CRL-7869 Hs 738.St/Int 人成纤维细胞
产品型号: CRL-7869
产品厂商: 美国标准生物品收藏中心(ATCC)
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CRL-7869 Hs 738.St/Int 人成纤维细胞,原代细胞|细胞系|细胞株|肿瘤细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!


CRL-7869 Hs 738.St/Int 人成纤维细胞 的详细介绍

CRL-7869 Hs 738.St/Int 人成纤维细胞

ATCC® Number: CRL-7869™    Price:  
Designations: Hs 738.St/Int
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)CRL-7869 Hs 738.St/Int 人成纤维细胞
Morphology: fibroblast

Source: Organ: mixed; stomach; intestine
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,11
D16S539: 9,11
D5S818: 11,13
D7S820: 8,12
THO1: 7,9.3
TPOX: 8
vWA: 16,18
Cytogenetic Analysis: modal number = 46; range = 44 to 92
Age: 18 weeks gestation fetus
Gender: CRL-7869 Hs 738.St/Int 人成纤维细胞male
Ethnicity: Caucasian
Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor termperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
CRL-7869 Hs 738.St/Int 人成纤维细胞

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