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NCI-H508 人结肠直肠腺癌

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产品名称: NCI-H508 人结肠直肠腺癌
产品型号: CCL-253
产品厂商: 美国标准生物品收藏中心(ATCC)
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CCL-253 NCI-H508 人结肠直肠腺癌,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


NCI-H508 人结肠直肠腺癌 的详细介绍
CCL-253 NCI-H508 人结肠直肠腺癌
ATCC® Number: CCL-253™    Price:  
Designations: NCI-H508 [H508]
Depositors:  AF Gazdar
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: floating aggregates of round cells with some attached cells
Organism: Homo sapiens (human)
Morphology: epithelial

Source: Organ: cecum
Disease: colorectal adenocarcinoma
Cellular Products: carcinoembryonic antigen (CEA), 1709 ng/ml per 10 exp6 cells per 10 days
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 CCL-253 NCI-H508 人结肠直肠腺癌
Tumorigenic: Yes
Antigen Expression: Blood type A; Rh+; CA19-9 antigen
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12
D5S818: 12
D7S820: 8,12
THO1: 9,9.3
TPOX: 8,11
vWA: 15,16
Cytogenetic Analysis: modal number = 102; range = 71 to 131; most cells have DM's
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 55 years
Gender: male
Ethnicity: Caucasian
Comments: This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil.
The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for two minutes. Remove the trypsin and let the flask sit at room temperature for 10 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249
CCL-253 NCI-H508 人结肠直肠腺癌

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