产品资料
产品[

CHO/dhFr- 仓鼠卵巢细胞,二氢叶酸还原酶缺陷

]资料
如果您对该产品感兴趣的话,可以 sendmsg
产品名称: CHO/dhFr- 仓鼠卵巢细胞,二氢叶酸还原酶缺陷
产品型号: CRL-9096
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


简单介绍

CHO/dhFr- 仓鼠卵巢细胞,二氢叶酸还原酶缺陷,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


CHO/dhFr- 仓鼠卵巢细胞,二氢叶酸还原酶缺陷 的详细介绍

CHO/dhFr- 仓鼠卵巢细胞,二氢叶酸还原酶缺陷

ATCC® Number:  CRL-9096™     
Designations:  CHO/dhFr- [CHO duk-, NOTE: dhfr refers to dihydrofolate reductase] 
Depositors:   Hoffmann-La Roche Ltd. 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Cricetulus griseus (hamster, Chinese) 
Morphology: epithelial

 
Source: Organ: ovary
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Gender:  female 
Propagation:  ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.1 mM hypoxanthine, 0.016 mM thymidine, 0.002mM Methotrexate (Amethopterin) and 10% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: The cells are deficient in dihydrofolate reductase.They should die in the absence of HT ( Hypoxanthine-Thymidine). Methotrexate (Amethopterin) is added to the cell culture medium to prevent growth of revertant cells with a low resistance to the drug.
Subculturing:  Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
Medium additive:ATCC 71-X
References: 26539: Shulman T, et al. An antibody reactive with domain 4 of the platelet-derived growth factor beta receptor allows BB binding while inhibiting proliferation by impairing receptor dimerization. J. Biol. Chem. 272: 17400-17404, 1997. PubMed: 9211881
30382: Morel-Kopp MC, et al. A three amino acid deletion in glycoprotein IIIa is responsible for type I glanzmann's thrombasthenia: importance of residues I1e325Pro326Gly327 for beta 3 integrin subunit association. Blood 90: 669-677, 1997. PubMed: 9226167
30522: Libyh MT, et al. A recombinant human scFv anti-Rh(D) antibody with multiple valences using a C-terminal fragment of C4-binding protein. Blood 90: 3978-3983, 1997. PubMed: 9354666
 Related Links  
NCBI Entrez Search 
Cell Micrograph 
 
 
Make a Deposit 
Frequently Asked Questions 
Material Transfer Agreement 
Technical Support 
Related Cell Culture Products  
 
 
 

产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

联系我们
产品搜索

细胞系,ATCC细胞,ATCC菌种,细胞系目录,ATCC,鼠伤寒沙门氏菌,atcc细胞库,人淋巴瘤细胞,人食管癌细胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌种,细胞株,标准菌株,ATCC中国,atcc上海代理,CMCC 菌株,ATCC菌株,细胞目录,质控菌株,肿瘤细胞株,乳腺癌细胞系,人肝癌细胞系

产品目录

Copyright© 2003-2019  上海复祥生物科技有限公司版权所有            电话:021-35080510
传真:021-35080520    地址:上海市虹口区赤峰路630号南雁大厦1901室    邮编: 沪ICP备10013034号-3  
  

沪公网安备 31010902002429号