产品资料
产品[

CHOK1仓鼠卵巢细胞亚株

]资料
如果您对该产品感兴趣的话,可以 sendmsg
产品名称: CHOK1仓鼠卵巢细胞亚株
产品型号: CRL-9618
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


简单介绍

CRL-9618 CHO-K1 仓鼠卵巢细胞亚株,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


CHOK1仓鼠卵巢细胞亚株 的详细介绍

CRL-9618 CHO-K1 仓鼠卵巢细胞亚株
ATCC® Number:  CRL-9618™       
Designations:  CHO-K1 
Depositors:   K Thomae 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Cricetulus griseus (hamster, Chinese) 
Morphology: epithelial

 
Source: Organ: ovary
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Applications: transfection host (Roche FuGENE® Transfection Reagents
Nucleofection technology from Lonza)
Gender:  female 
Comments: This is the same line as ATCC CCL-61.
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:  Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Once or twice between subculture
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation:  culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020 
 
 

产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

联系我们
产品搜索

细胞系,ATCC细胞,ATCC菌种,细胞系目录,ATCC,鼠伤寒沙门氏菌,atcc细胞库,人淋巴瘤细胞,人食管癌细胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌种,细胞株,标准菌株,ATCC中国,atcc上海代理,CMCC 菌株,ATCC菌株,细胞目录,质控菌株,肿瘤细胞株,乳腺癌细胞系,人肝癌细胞系

产品目录

Copyright© 2003-2019  上海复祥生物科技有限公司版权所有            电话:021-35080510
传真:021-35080520    地址:上海市虹口区赤峰路630号南雁大厦1901室    邮编: 沪ICP备10013034号-3  
  

沪公网安备 31010902002429号