CRFK 猫肾细胞
ATCC® Number: CCL-94™
Designations: CRFK
Depositors: RA Van Deusen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Felis catus (cat)
Morphology: epithelial
Source: Organ: kidney, cortex
Disease: normal
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Applications: transfection host (Roche FuGENE® Transfection Reagents)
Virus Susceptibility: feline herpesvirus; feline calicivirus; feline panleukopenia; feline reovirus; canine parvovirus; rabiesvirus; bovine viral diarrhea (BVD) virus
Reverse Transcript: negative
Age: 12 weeks
Gender: female
Comments: The cells are free of bovine viral diarrhea (BVD) virus.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin 0.02% EDTA solution for 1 to 2 minutes, remove trypsin and allow culture to stand at room temperature (or at 37C) until cells detach . Add fresh medium, aspirate and dispense into new flasks.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
References: 26266: Crandell RA, et al. Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK). In Vitro 9: 176-185, 1973. PubMed: 4130570
26267: Fabricant CG, Rich LJ. Microbial studies of feline urolithiasis. J. Am. Vet. Med. Assoc. 158: 976-980, 1971. PubMed: 4930310
26268: Lee KM, et al. Use of an established cat cell line for investigation and quantitation of feline oncornaviruses. J. Natl. Cancer Inst. 49: 55-60, 1972. PubMed: 4338781
28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321
