CCL-89 AtT-20 小鼠垂体瘤细胞
ATCC® Number: CCL-89™
Designations: AtT-20
Depositors: G Sato
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Organism: Mus musculus (mouse)
Morphology: small rounded cells
Source: Organ: pituitary
Strain: LAF1
Disease: tumor
Cellular Products: adrenocorticotropic hormone (ACTH)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: October, 1966
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Virus Susceptibility: Vesicular stomatitis virus
Human poliovirus 1
Reverse Transcript: positive
Comments: Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Temperature: 37.0°C
Growth Conditions: The cells do not attach but aggregate to form clusters; the clusters are viable.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. Proliferation is enhanced by incubation on a shaking platform.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Preservation: Freeze medium: culture medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
derivative:ATCC CRL-1795
References: 23251: Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682
26257: Furth J, et al. ACTH secreting transplantable pituitary tumors. Proc. Soc. Exp. Biol. Med. 84: 253-254, 1953. PubMed: 13121002
28712: Tabernero C, et al. Identification of an RNA sequence within an intracisternal-A particle element able to replace Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1. J. Virol. 71: 95-101, 1997. PubMed: 8985327
33043: Gamby C, et al. Growth-associated protein-43 (GAP-43) facilitates peptide hormone secretion in mouse anterior pituitary AtT-20 cells. J. Biol. Chem. 271: 10023-10028, 1996. PubMed: 8626556
