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CCL-247 HCT 116 人结肠癌细胞

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产品名称: CCL-247 HCT 116 人结肠癌细胞
产品型号: CCL-247
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


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CCL-247 HCT 116 人结肠癌细胞,原代细胞|细胞系|细胞株|菌种,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件!


CCL-247 HCT 116 人结肠癌细胞 的详细介绍

CCL-247 HCT 116 人结肠癌细胞
ATCC® Number:  CCL-247™     
Designations:  HCT 116 
Depositors:   MG Brattain 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Homo sapiens (human) 
Morphology: epithelial

 
Source: Organ: colon
Disease: colorectal carcinoma
Cellular Products: carcinoembryonic antigen (CEA) 1 ng per 10 exp6 cells per 10 days; keratin 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Tumorigenic: Yes 
Reverse Transcript: negative 
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 10,12
D16S539: 11,13
D5S818: 10,11
D7S820: 11,12
THO1: 8,9
TPOX: 8,9
vWA: 17,22
Cytogenetic Analysis: The stemline chromosome number is near diploid with the modal number at 45 (62%) and polyploids occurring at 6.8%. The markers 10q+ and t(?8p;18q) are present in all metaphases and t(9q;?16p-), in 80% of the cells karyotyped. N16 is monosomic in the presence of, but disomic in the absence of t(9q;?16p-). N10 and N18 are monosomic and other chromosomes from those mentioned above are disomic. Q-band observations revealed the presence of the Y chromosome, but not in all cells (50% of cells lacked the Y in G-band karyotypes).
Isoenzymes:  AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 1
PGM1, 1
PGM3, 1

Gender:  male 
Comments: The cells are positive for keratin by immunoperoxidase staining.
HCT 116 cells are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression.
This line has a mutation in codon 13 of the ras protooncogene, and can be used as a positive control for PCR assays of mutation in this codon.
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts.
Subculturing:  Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

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