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WPE-int
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MM Webber, EJ Tokar
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Biosafety Level:
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2 [cells contain human HPV-18 viral DNA sequences ]
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frozen
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Medium & Serum:
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial
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Organ: prostate, normal, peripheral zone
Cell Type: epithelial
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Isolation date: 2004 [PubMed: 16351690] [92794]
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androgen receptor, expressed ( [92794] upregulated upon exposure to androgen)
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NO
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kallikrein 3 ( KLK3); prostate specific antigen (PSA); expressed (upregulated upon exposure to androgen) [PubMed: 16351690] [92794]
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Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,14
D16S539: 9,11
D5S818: 12,15
D7S820: 10,11
THO1: 8
TPOX: 8,11
vWA: 14,18
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54
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male
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
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0.05 mg/ml BPE - provided with the K-SFM kit
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5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.
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