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CCL-246 KG-1 人白血病细胞

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产品名称: CCL-246 KG-1 人白血病细胞
产品型号: CCL-246
产品厂商: 美国标准生物品收藏中心(ATCC)
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CCL-246 KG-1 人白血病细胞,原代细胞|细胞系|细胞株|菌种,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件!


CCL-246 KG-1 人白血病细胞 的详细介绍

CCL-246 KG-1 人白血病细胞
ATCC® Number:  CCL-246™      
Designations:  KG-1 
Depositors:   DW Golde 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: suspension
Organism: Homo sapiens (human) 
Morphology: myeloblast

 
Source: Organ: bone marrow
Disease: acute myelogenous leukemia
Cellular Products: HLA DR 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Applications: transfection host (technology from amaxa)
Reverse Transcript: negative 
Antigen Expression: HLA A30, A31, B35, Cw4
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Cytogenetic Analysis: The stemline chromosome number is near-diploid, with the 2S component occurring at 1.8%. Five markers (constitutive markers) were common to most, if not all, metaphases analyzed. Modal chromosome number is 47 (or 46 plus a small metacentric chromosome which is smaller than the G1 group chromosome). Normal chromosomes 5, 7, 8, 12 and 17 were monosomic, and others were disomic. The Y chromosome is detected in the Q-banded preparations.
Isoenzymes:  AK-1, 0
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1-2
PGM3, 0
Age:  59 years 
Gender:  male 
Ethnicity:  Caucasian 
Comments: The KG-1 cell line was derived by H.P. Koeffler and D.W. Golde. A bone marrow aspirate was obtained from a 59-year-old Caucasian male with erythroleukemia that evolved into acute myelogenous leukemia. [1056]
KG-1 cells spontaneously differentiate to granulocyte and macrophage like cells.
They show a good response to colony stimulating factor (CSF). [1056]
The line is EBNA negative (EBNA-).
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing:  Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 (5) viable cells/ml. Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml. Do not allow to exceed 2 X 10(6) cells/ml.
Medium Renewal: Twice per week
Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 867: Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765
1056: Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682
26069: . . Blood 54: 174a, 1979.
33055: Penrose JF, et al. Molecular cloning of the gene for human leukotriene C4 synthase. J. Biol. Chem. 271: 11356-11361, 1996. PubMed: 8626689
 Related Links  
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