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CCL-7 LLC-MK2 恒河猴肾细胞

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产品名称: CCL-7 LLC-MK2 恒河猴肾细胞
产品型号: CCL-7
产品厂商: 美国标准生物品收藏中心(ATCC)
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CCL-7 LLC-MK2 恒河猴肾细胞,原代细胞|细胞系|细胞株|菌种,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!


CCL-7 LLC-MK2 恒河猴肾细胞 的详细介绍

CCL-7 LLC-MK2 恒河猴肾细胞
ATCC® Number:  CCL-7™      
Designations:  LLC-MK2 Original 
Depositors:   RN Hull 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Macaca mulatta (monkey, rhesus) 
Morphology: epithelial

 
Source: Organ: kidney
Disease: normal
Cellular Products: plasminogen activator 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Virus Susceptibility: poliovirus 1; coxsackievirus B4; bovine viral diarrhea (BVD) virus
Reverse Transcript: negative 
Age:  
Comments: The serum used in the medium should be pre-tested for good quality.
Some lots of horse serum do support satisfactory growth.
Adaptation to other types of sera generally results in slow growth with a prolonged lag in growth.
Propagation:  ATCC complete growth medium: Medium 199 (with 1.68 g/L bicarbonate), 99%; horse serum, 1%
Temperature: 37.0°C
Subculturing:  Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Preservation:  Culture medium, 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2040
References: 22310: . . Anat. Rec. 124: 490, 1956.
26049: Hull RN, et al. Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research. J. Exp. Med. 115: 903-918, 1962. PubMed: 14449901
26065: Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902 
 
 

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