CRL-2192 NR8383 大鼠肺泡巨噬细胞
ATCC® Number: CRL-2192™
Designations: NR8383 [AgC11x3A, NR8383.1]
Depositors: RJ Helmke
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed, adherent and suspension
Organism: Rattus norvegicus (rat)
Morphology: macrophage
Source: Organ: lung
Strain: Sprague-Dawley
Disease: normal
Cell Type: macrophage (alveolar);
Cellular Products: transforming growth factor beta (TGF beta); interleukin 1 (IL-1); interleukin 6 (IL-6)
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Isolation: Isolation date: August 3, 1983
Receptors: Fc
Comments: NR8383 (normal rat, August 3, 1983) was established from normal rat alveolar macrophage cells obtained by lung lavage.
The cells were cultured in the presence of gerbil lung cell conditioned medium for approximately 8 to 9 months.
Subsequently the requirement for exogenous growth factors was lost.
NR8383 cells were cloned and subcloned from single cells by limiting dilution, and then subcloned from soft agar three times.
The cells exhibit characteristics of macrophage cells:
Phagocytosis of zymosan and Pseudomonas aeruginosa, nonspecific esterase activity, Fc receptors, oxidative burst,IL-1, TNF beta and IL-6 secretion, and replicative response to exogenous growth factors.
The cells respond to appropriate microbial, particulate or soluble stimuli with phagocytosis and killing.
NR8383 cells respond to bleomycin by secreting latent transforming growth factor (TGF beta).
Stimulation with bleomycin also increases TGF beta mRNA expression.
These cells are sensitive to endotoxin.
LPS levels of 1 to 10 ng/ml inhibit replication by 50%.
LPS inhibition is nontoxic and reversible even after levels up to 0.001 mg/ml for extended periods.
The NR8383 cell line provides a homogenous source of highly responsive alveolar macarophages which can be used in vitro to study macrophage related activities.
Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; heat inactivated fetal bovine serum, 15%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by transferring floating cells to additional flasks. Adherent cells may be harvested by scraping. Upon reseeding, about one half of the cells will re-attach. Cultures are most successful when set up at a floating cell concentration of 1 to 4 X 10 exp5 viable cells/ml.
Medium Renewal: Two to three times weekly
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
purified RNA:ATCC CRL-2192R
References: 22160: Hidalgo HA, et al. Pneumocystis carinii induces an oxidative burst in alveolar macrophages. Infect. Immun. 60: 1-7, 1992. PubMed: 1729174
22316: Helmke RJ, et al. A continuous alveolar macrophage cell line: comparisons with freshly derived alveolar macrophages. In Vitro Cell. Dev. Biol. 25: 44-48, 1989. PubMed: 2914814
22674: Helmke RJ, et al. From growth factor dependence to growth factor responsiveness: the genesis of an alveolar macrophage cell line. In Vitro Cell. Dev. Biol. 23: 567-574, 1987. PubMed: 3497918
22848: Limper AH, Standing JE. Vitronectin interacts with Candida albicans and augments organism attachment to the NR8383 macrophage cell line. Immunol. Lett. 42: 139-144, 1994. PubMed: 7534269
22970: Hidalgo HA, et al. The effects of cyclosporine and dexamethasone on an alveolar macrophage cell line (NR8383). Transplantation 53: 620-623, 1992. PubMed: 1549855
23173: Denholm EM, Rollins SM. Expression and secretion of transforming growth factor-beta by bleomycin-stimulated rat alveolar macrophages. Am. J. Physiol. 264: L36-L42, 1993. PubMed: 7679254
23190: Krieg DP, et al. Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophages in vitro. Infect. Immun. 56: 3173-3169, 1988. PubMed: 3141284
23369: Sherman MP, et al. Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages. Biochem. Biophys. Res. Commun. 191: 1301-1308, 1993. PubMed: 7682068
23484: Griscavage JM, et al. Inducible nitric oxide synthase from a rat alveolar macrophage cell line is inhibited by nitric oxide. J. Immunol. 151: 6329-6337, 1993. PubMed: 7504017
23566: Henderson SA, et al. Nitric oxide reduces early growth response-1 gene expression in rat lung macrophages treated with interferon-gamma and lipopolysaccharide. J. Biol. Chem. 269: 25239-25242, 1994. PubMed: 7523382
36466: Huang S, et al. Rat KC cDNA cloning and mRNA expression in lung macrophages and fibroblasts. Biochem. Biophys. Res. Commun. 184: 922-929, 1992. PubMed: 1374243
