CRL-1949 Psi2 DAP 小鼠胚胎成纤维细胞
ATCC® Number: CRL-1949™
Designations: Psi2 DAP
Depositors: CL Cepko
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: fibroblast
Source: Strain: NIH/Swiss
Organ: embryo
Disease: normal
Cellular Products: a human placental alkaline phosphatase transducing vector
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Reverse Transcript: positive
Age: embryo
Comments: This line produces a vector that can infect mouse cells.
Psi2 DAP cells produce a vector that encodes the human placental alkaline phosphatase gene and the neomycin resistance gene.
The line was derived from Psi2 cells by insertion of a recombinant retrovirus genome (DAP).
Cells infected with the vector produced by Psi2 DAP cells express histochemically detectable alkaline phosphatase which can be used as a marker to follow their fate in vivo.
The cells may produce helper virus.
To assay for helper virus, and for other retrovirus techniques, see Cepko, C.L.
Lineage analysis and immortalization of neural cells via retrovirus vectors in Neuromethods, Molecular Neurobiological Techniques 16:117-219, Boulton, A.A., Baker, G.B. and Campagnoni, A.T., Eds., Humana Press, Clifton, NJ, 1989.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:50 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks.
References: 22598: Fields-Berry SC, et al. A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Proc. Natl. Acad. Sci. USA 89: 693-697, 1992. PubMed: 1731342
