产品[
RF/6A 猴脉络膜-视网膜(内皮)细胞
]资料
如果您对该产品感兴趣的话,可以
产品名称:
RF/6A 猴脉络膜-视网膜(内皮)细胞
产品型号:
CRL-1780
产品厂商:
美国标准生物品收藏中心（ATCC）
产品文档:
无相关文档
简单介绍
CRL-1780 RF/6A 猴脉络膜-视网膜(内皮)细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
RF/6A 猴脉络膜-视网膜(内皮)细胞
的详细介绍
CRL-1780 RF/6A 猴脉络膜-视网膜(内皮)细胞
|
|
CRL-1780™
|
|
|
|
|
RF/6A
|
|
|
F Hu
|
|
Biosafety Level:
|
1
|
|
|
frozen
|
|
Medium & Serum:
|
See Propagation
|
|
|
adherent
|
|
|
Macaca mulatta (monkey, rhesus)
|
|
|
epithelial
|
|
|
Tissue: choroid; retina
Disease: normal
Cell Type: endothelial
|
|
|
factor VIII; fibronectin
|
|
|
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
|
|
|
|
|
fetus
|
|
|
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
|
|
|
Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 2 minutes at room temperature, remove trypsin and let the culture sit at 37C for 5 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice weekly
|
|
|
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
|
|
|
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
|
|
|
22315: Lou DA, Hu FN. Specific antigen and organelle expression of a long-term rhesus endothelial cell line. In Vitro Cell. Dev. Biol. 23: 75-85, 1987. PubMed: 3102454
|
|
|
