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HTB-35 SiHa 人子宫颈鳞癌细胞

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产品名称: HTB-35 SiHa 人子宫颈鳞癌细胞
产品型号: HTB-35
产品厂商: 美国标准生物品收藏中心(ATCC)
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HTB-35 SiHa 人**颈鳞癌细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


HTB-35 SiHa 人子宫颈鳞癌细胞 的详细介绍
HTB-35 SiHa 人**颈鳞癌细胞
ATCC® Number: HTB-35™    Price: $273.00
Designations: SiHa
Depositors:  Y Ito
Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial

Source: Organ: cervix
Tumor Stage: grade II
Disease: squamous cell carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic: Yes
Oncogene: p53 +; pRB +
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 12
D5S818: 9
D7S820: 10
THO1: 6,9
TPOX: 8
vWA: 14,17
Cytogenetic Analysis: modal number = 69; range = 51 to 72.
This is a hypertriploid human cell line with the modal chromosome number of 71, occurring in 24% of cells. Most cells had the chromosome numbers distributed between 69 and 72. Polyploid cells occurred at 7.6%. Fifteen or more marker chromosomes were common to most cells. Among them are dup(2) (q22q31) and del(2) (q31) which probably resulted from the balanced translocation between two N2s. Most cells had two copies of del(2). M2 is an A3-sized acrocentric. M13 is a minute submetacentric with 1-3 copies per cell. Origins of both M2 and M13 are not identified. There were two copies of normal X chromosomes. N2 was absent and probably was replaced by dup(2) and del(2).
Isoenzymes: AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 55 years *****
Gender: female
Ethnicity: Asian
Comments: This line was established from fragments of a primary tissue sample obtained after surgery from a Japanese patient.
Electron microscopic observations revealed presence of typical desmosomes at the cell junctions and an abundance of tonofilaments in the cytoplasm.
Mycoplasma contamination was detected and eliminated in 1975.
The line is reported to contain an integrated human papillomavirus type 16 genome (HPV-16, 1 to 2 copies per cell).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
   
   
   
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