产品资料
产品[

HTB-52 SK-HEP-1 人肝癌细胞

]资料
如果您对该产品感兴趣的话,可以 sendmsg
产品名称: HTB-52 SK-HEP-1 人肝癌细胞
产品型号: HTB-52
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


简单介绍

HTB-52 SK-HEP-1 人肝癌细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


HTB-52 SK-HEP-1 人肝癌细胞 的详细介绍
HTB-52 SK-HEP-1 人肝癌细胞
ATCC® Number: HTB-52™    Price: $329.00
Designations: SK-HEP-1
Depositors:  G Trempe, LJ Old
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial

Source: Organ: liver
Tissue: ascites
Disease: adenocarcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Isolation: Isolation date: 1971
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12
D5S818: 10,13
D7S820: 8,11
THO1: 7,9
TPOX: 9
vWA: 14,17
Cytogenetic Analysis: The cell line is aneuploid human (XX), with chromosome counts in the hypotriploid range. Normal chromosomes N1, N13, N14, N15, and N16 are clearly under-represented with respect to the copy number of other normal chromosomes, while chromosome N7, N12, and N17 tend to be over-represented in some metaphases. Eight markers are identified: del(1)(q21), der(1)t(1;2)(p36;q21), ?tdic(1;12)(p36;q24), ampl.t(4pter--->4q11::HSR::4q12--->4q24::HSR?::4q26--->4q35::2q15--->2pter), 13p+, 13p++, 14p+, t(14q15q), iso(14q), 19q+, del(3)(p14p24), 15p+.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 1-2
PGM1, 2
PGM3, 1
Age: 52 years
Gender: male
Ethnicity: Caucasian
Comments: The SK-HEP-1 line has been identified as being of endothelial origin.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSOComplete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
24396: Heffelfinger SC, et al. SK HEP-1: a human cell line of endothelial origin. In Vitro Cell. Dev. Biol. 28A: 136-148, 1992. PubMed: 1371504
56315: Gucev ZS, et al. Evidence for insulin-like growth factor (IGF)-independent transcriptional regulation of IGF binding protein-3 by growth hormone in SKHEP-1 human hepatocarcinoma cells. Endocrinology 138: 1464-1470, 1997. PubMed: 9075703
58075: Turner BM, Turner VS. Secretion of alpha1-antitrypsin by an established human hepatoma cell line and by human/mouse hybrids. Somatic Cell Genet. 6: 1-14, 1980. PubMed: 6245472
产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

联系我们
产品搜索

细胞系,ATCC细胞,ATCC菌种,细胞系目录,ATCC,鼠伤寒沙门氏菌,atcc细胞库,人淋巴瘤细胞,人食管癌细胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌种,细胞株,标准菌株,ATCC中国,atcc上海代理,CMCC 菌株,ATCC菌株,细胞目录,质控菌株,肿瘤细胞株,乳腺癌细胞系,人肝癌细胞系

产品目录

Copyright© 2003-2019  上海复祥生物科技有限公司版权所有            电话:021-35080510
传真:021-35080520    地址:上海市虹口区赤峰路630号南雁大厦1901室    邮编: 沪ICP备10013034号-3  
  

沪公网安备 31010902002429号