产品资料
产品[

HTB-4 T24 人膀胱移行细胞癌细胞

]资料
如果您对该产品感兴趣的话,可以 sendmsg
产品名称: HTB-4 T24 人膀胱移行细胞癌细胞
产品型号: HTB-4
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


简单介绍

HTB-4 T24 人膀胱移行细胞癌细胞, ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


HTB-4 T24 人膀胱移行细胞癌细胞 的详细介绍
HTB-4 T24 人膀胱移行细胞癌细胞
ATCC® Number: HTB-4™   
Designations: T24
Depositors:  C O'Toole
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial

Source: Organ: urinary bladder
Disease: transitional cell carcinoma
Cellular Products: tumor specific antigen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (Roche FuGENE® Transfection Reagents
technology from amaxa)
Tumorigenic: Yes
Antigen Expression: HLA A1, A3, B18, Bw35, Cw4, DRw2, Dw4
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 12
D16S539: 9
D5S818: 10,12
D7S820: 10,11
THO1: 6
TPOX: 8,11
vWA: 17
Cytogenetic Analysis: hypodiploidy to hypopentaploidy; stemline 86; 2 to 4 telocentrics; 3 to 4 minutes, hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, pulverization, minutes and telocentric markers
Comments: Leukocytes and sera from patients with transitional cell carcinoma were cytotoxic to T24 and related lines.
Cells have a 19 hour generation time.
Contains the ras (H-ras) oncogene.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
References: 21849: O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.
22365: O'Toole C, et al. Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int. J. Cancer 10: 77-91, 1972. PubMed: 4196436
22443: Williams BY, Schonbrunn A. Bombesin receptors in a human duodenal tumor cell line: binding properties and function. Cancer Res. 54: 818-824, 1994. PubMed: 8306345
22510: Bubenik J, et al. Cellular and humoral immune responses to human urinary bladder carcinomas. Int. J. Cancer 5: 310-319, 1970. PubMed: 5452065
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
22849: Bubenik J, et al. Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen. Int. J. Cancer 11: 765-773, 1973. PubMed: 4133950
23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
23256: Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619
24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484
26316: Bubenik J, et al. Cellular immunity to renal carcinomas in man. Int. J. Cancer 8: 503-513, 1971. PubMed: 5137312
32266: Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064
33025: Ponton A, et al. The CD95 (APO-1/Fas) receptor activates NF-kappaB independently of its cytotoxic function. J. Biol. Chem. 271: 8991-8995, 1996. PubMed: 8621545
产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

联系我们
产品搜索

细胞系,ATCC细胞,ATCC菌种,细胞系目录,ATCC,鼠伤寒沙门氏菌,atcc细胞库,人淋巴瘤细胞,人食管癌细胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌种,细胞株,标准菌株,ATCC中国,atcc上海代理,CMCC 菌株,ATCC菌株,细胞目录,质控菌株,肿瘤细胞株,乳腺癌细胞系,人肝癌细胞系

产品目录

Copyright© 2003-2019  上海复祥生物科技有限公司版权所有            电话:021-35080510
传真:021-35080520    地址:上海市虹口区赤峰路630号南雁大厦1901室    邮编: 沪ICP备10013034号-3  
  

沪公网安备 31010902002429号