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CCL-81 Vero 非洲绿猴肾细胞

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产品名称: CCL-81 Vero 非洲绿猴肾细胞
产品型号: CCL-81
产品厂商: 美国标准生物品收藏中心(ATCC)
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CCL-81 Vero 非洲绿猴肾细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


CCL-81 Vero 非洲绿猴肾细胞 的详细介绍
CCL-81 Vero 非洲绿猴肾细胞
ATCC® Number: CCL-81™    Price:
Designations: Vero
Depositors:  W Hann, JS Rhim
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties:adherent
Organism:Cercopithecus aethiops
Morphology:epithelial
CCL-81 Vero 非洲绿猴肾细胞
Source:Organ: kidney
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Isolation: Isolation date: March 27, 1962
Applications:detection of verotoxin [21447]
efficacy testing [92579] [92587]
malaria biology
media testing [11019]
mycoplasma testing [92577]
substrate [92447]
testing [34219] [92309] [92319] [92320] [92321] [92322] [92324] [92346] [92389]
transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
detection of virus in ground beef [34219]
Virus Susceptibility:poliovirus 1, 2, 3; Getah; Ndumu; Pixuna; Ross River; Semliki Forest; Paramaribo; Kokobera; Modoc; Murutucu; Germiston; Guaroa; Pongola; Tacaribe; SV-5; SV40; rubeola; rubellavirus; reovirus 1, 2, 3; simian adenoviruses
Virus Resistance:Stratford; Apeu; Caraparu; Madrid; Nepuyo; Ossa
Reverse Transcript:negative
Cytogenetic Analysis:This is a cell line with the hypodiploid chromosome count. The modal chromosome number was 58 occurring in 66% of cells. In most cells, over 50% of the chromosomes in each cell complement belonged to structurally altered marker chromosomes. Normal A3, A4, B4, and B5 were absent; B2, B3 and B7 were occasionally paired; and B9, C1 and C5 were mostly paired. The rate of cells with higher ploidies was 1.7%. Other chromosomes were mostly present in single copy.
Age: *****
Comments:The Vero cell line was initiated from the kidney of a normal ***** African green monkey on March 27, 1962, by Y. Yasumura and Y. Kawakita at the Chiba University in Chiba, Japan. [21447]
The cell line was brought to the Laboratory of Tropical Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health in the 93rd passage from Chiba University by B. Simizu on June 15, 1964.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
derivative:ATCC CRL-1587
References: 
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