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WI-38 人胚肺细胞

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产品名称: WI-38 人胚肺细胞
产品型号: CCL-75
产品厂商: 美国标准生物品收藏中心(ATCC)
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CCL-75 WI-38 人胚肺细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!


WI-38 人胚肺细胞 的详细介绍
CCL-75 WI-38 人胚肺细胞
ATCC® Number: CCL-75™    Price: $272.00
Designations: WI-38
Depositors:  L Hayflick
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: fibroblast

Source: Organ: lung
Disease: normal
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: testing [92346] [92389]
transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
viruscide testing
Virus Susceptibility: Vesicular stomatitis, Glasgow (Indiana)
Herpes simplex virus
Pseudorabies virus
Human poliovirus 1
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20
Cytogenetic Analysis: normal diploid
Isoenzymes: G6PD, B
Age: 3 months gestation fetus
Gender: female
Ethnicity: Caucasian
Comments: The WI-38 human diploid cell line was derived by Leonard Hayflick from normal embryonic (3 months gestation) lung tissue. [22194]
WI-38 cells have a finite lifetime of 50 plus or minus 10 population doublings with a doubling time of 24 hours. [22197]
This line was the first human diploid cell line to be used in human vaccine preparation. [26220]
The 8th passage ampule from which this freeze was derived was found to contain a bacterial contaminant (a micrococcus). The cell line was subsequently cured by several passages in the presence of antibiotics.
Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Trypsin and medium should be prewarmed to 37C and the pH should be adjusted to 7.5 before use.
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: growth medium, 95%; DMSO, 5%
Doubling Time: 24 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 21405: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111
22194: Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585-621, 1961. PubMed: 13905659
22197: Hayflick L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614-636, 1965. PubMed: 14315085
26220: Hayflick L, et al. Preparation of poliovirus vaccines in a human fetal diploid cell strain. Am. J. Hyg. 75: 240-258, 1962. PubMed: 13905660
26223: . First International Conference on Vaccines against Viral and Rickettsial Diseases of Man. Pan Am. Health Organ. Off. Doc. 147: 581, 1967.
29375: Tomasek JJ, et al. Gelatinase A activation Is regulated by the organization of the polymerized actin cytoskeleton. J. Biol. Chem. 272: 7482-7487, 1997. PubMed: 9054450
32244: Hoppe HC, et al. Identification of phosphatidylinositol mannoside as a mycobacterial adhesin mediating both direct and opsonic binding to nonphagocytic mammalian cells. Infect. Immun. 65: 3896-3905, 1997. PubMed: 9284169
32288: Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029
32582: Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591
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