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A549人肺癌细胞

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产品名称: A549人肺癌细胞
产品型号: CCL-185
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


简单介绍

CCL-185 A549 人肺癌细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


A549人肺癌细胞 的详细介绍
CCL-185 A549 人肺癌细胞
ATCC® Number: CCL-185™    Price: $256.00
Designations: A549
Depositors:  M Lieber
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
CCL-185 A549 人肺癌细胞
Source: Organ: lung
Disease: carcinoma
Cellular Products: keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Isolation: Isolation date: 1972
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14
Cytogenetic Analysis: This is a hypotriploid human cell line with the modal chromosome number of 66, occurring in 24% of cells. Cells with 64 (22%), 65, and 67 chromosome counts also occurred at relatively high frequencies; the rate with higher ploidies was low at 0.4%. There were 6 markers present in single copies in all cells. They include der(6)t(1;6) (q11;q27); ?del(6) (p23); del(11) (q21), del(2) (q11), M4 and M5. Most cells had two X and two Y chromosomes. However, one or both Y chromosomes were lost in 40% of 50 cells analyzed. Chromosomes N2 and N6 had single copies per cell; and N12 and N17 usually had 4 copies.
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