CRL-1444 大鼠胸大动脉平滑肌细胞

]资料

点击看大图

如果您对该产品感兴趣的话,可以

产品名称: CRL-1444 大鼠胸大动脉平滑肌细胞
产品型号: A7r5
产品厂商: 美国标准生物品收藏中心（ATCC）
产品文档: 无相关文档

简单介绍

CRL-1444 A7r5 大鼠胸大动脉平滑肌细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件!

CRL-1444 大鼠胸大动脉平滑肌细胞的详细介绍

CRL-1444 A7r5 大鼠胸大动脉平滑肌细胞

ATCC^® Number:

CRL-1444™

Price:

$275.00

Designations:	A7r5
Depositors:	W Carlisle
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Rattus norvegicus (rat)
Morphology:	fibroblast
Source:	Organ: aorta, thoracic Strain: DB1X Tissue: smooth muscle
Cellular Products:	myokinase; creatine phosphokinase (muscle isoenzyme); myosin
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:	transfection host (Roche FuGENE® Transfection Reagents technology from amaxa)
Age:	embryo
Comments:	The cells exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase (CPK) as the culture reaches stationary phase. Muscle type CPK is synthesized after cell division has ceased.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days
Preservation:	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020
References:	1061: Kimes BW, Brandt BL. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res. 98: 349-366, 1976. PubMed: 943301

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

细胞系，ATCC细胞，ATCC菌种，细胞系目录，ATCC，鼠伤寒沙门氏菌，atcc细胞库，人**瘤细胞，人食管癌细胞，ATCC 19404，ATCC 25922，ATCC 19146，微生物菌种，细胞株，标准菌株，ATCC中国，atcc上海代理，CMCC 菌株，ATCC菌株，细胞目录，质控菌株，肿瘤细胞株，乳腺癌细胞系，人肝癌细胞系

产品目录