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  • 产品名称：BALB/3T3 clone A31小鼠胚胎成纤维细胞

  • 产品型号：CCL-163A31小鼠胚胎成纤维细胞
  • 产品厂商：美国标准生物品收藏中心（ATCC）
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BALB/3T3 clone A31小鼠胚胎成纤维细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件!BALB/3T3 clone A31小鼠胚胎成纤维细胞
详情介绍：
BALB/3T3 clone   A31小鼠胚胎成纤维细胞
ATCC® Number: CCL-163™    Price: $269.00
Designations: BALB/3T3 clone A31
Depositors:  S Aaronson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: fibroblast
BALB/3T3 clone A31小鼠胚胎成纤维细胞
Source: Organ: embryo
Strain: BALB/c
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Isolation: Isolation date: 1968
Applications: testing [92346] [92389]
transfection host (Roche FuGENE® Transfection Reagents)
Virus Susceptibility: Herpes simplex virus
Vesicular stomatitis virus
Human poliovirus 1
Tumorigenic: No
Reverse Transcript: negative
Cytogenetic Analysis: modal number = 78; range = 62 to 109.
The stemline number is hypotetraploid. Most of the cells contained only telocentric or acrocentric chromosomes.
Age: embryo; 14 to 17 day gestation
Comments: The BALB/3T3 clone A31 is one of several cell lines (see ATCC CCL-164, BALB/3T12) developed by S.A. Aaronson and G.T. Todaro in 1968 from disaggregated 14- to 17-day-old BALB/c mouse embryos [PubMed: 4301006].The cells are extremely sensitive to contact inhibition of cell division, grow at a high dilution, exhibit a low saturation density and are highly susceptible to transformation in tissue culture by the oncogenic DNA virus SV40 and murine sarcoma virus [PubMed: 4306523].
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: The serum used is calf serum, NOT fetal calf serum. The depositor recommended calf serum because fetal calf serum causes transformation and loss of contact inhibition.
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