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CRL-1772 C2C12 小鼠成肌细胞

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产品名称: CRL-1772 C2C12 小鼠成肌细胞
产品型号: CRL-1772
产品厂商: 美国标准生物品收藏中心(ATCC)
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CRL-1772 C2C12 小鼠成肌细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


CRL-1772 C2C12 小鼠成肌细胞 的详细介绍
CRL-1772 C2C12 小鼠成肌细胞
ATCC® Number: CRL-1772™    Price: $256.00
Designations: C2C12
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: myoblast
CRL-1772 C2C12 小鼠成肌细胞
Source: Strain: C3H
Tissue: muscle
Cell Type: myoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Comments: This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903]
The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953]
Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427]
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT.
Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture.
Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
    Inoculate at a cell concentration between 1.5 X 10 exp5 and 1.0 X 10 exp6 viable cells/75 cm2.
  6. Incubate cultures at 37°C.

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