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  • 产品名称： Capan-2 人胰腺癌细胞

  • 产品型号：HTB-80
  • 产品厂商：美国标准生物品收藏中心（ATCC）
  • 产品价格：0
  • 折扣价格：0
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简单介绍：
HTB-79 Capan-2 人胰腺癌细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
详情介绍：
HTB-79 Capan-2 人胰腺癌细胞
ATCC® Number: HTB-80™    Price: $269.00
Designations: Capan-2
Depositors:  J Fogh
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: pancreas
Disease: adenocarcinoma
Derived from metastatic site: liver
Cellular Products: mucin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: The cells will slough off of the growth surface if they become too heavy.
Capan-1 expresses the cystic fibrosis transmembrane conductance regulator (CFTR) and secrete gastric type mucins.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
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