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CTLL-2 小鼠T细胞 IL-2依赖型

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产品名称: CTLL-2 小鼠T细胞 IL-2依赖型
产品型号: TIB-214
产品厂商: 美国标准生物品收藏中心(ATCC)
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CTLL-2 小鼠T细胞IL-2依赖型,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


CTLL-2 小鼠T细胞 IL-2依赖型 的详细介绍
CTLL-2  小鼠T细胞IL-2依赖型
ATCC® Number: TIB-214™    Price: $289.00
Designations: CTLL-2
Depositors:  S Gillis
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (mouse)
Morphology: lymphoblast
CTLL-2 小鼠T细胞 IL-2依赖型
Source: Cell Type: lymphocyte cytotoxic T lymphocyte;
Strain: C57BL/6
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Receptors: interleukin 2 (IL-2), expressed
Comments: This line is a clone of cytotoxic T cells derived from a C57BL/6 mouse. The cells are dependent upon IL-2 for growth, and can be used to assay for IL-2.Immediately after thawing (or after overgrowth of a culture) the culture will appear to have few or no viable cells. This is normal.Depending upon the source and potency of the IL-2, it may take up to three weeks before the cells are ready to subculture.T-STIM with Con A (rat IL-2 culture supplement from Becton Dickinson) may be used or the rat factor may be prepared as described on the product sheet. The freshly prepared rat factor will usually produce more rapid growth.Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: additional 2 mM L-glutamine; additional 1mM sodium pyruvate; adjust to a final concentration of 10% fetal bovine serum and 10% T-STIM with Con A. T-STIM is available from Becton Dickinson.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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