产品资料
产品[

CV-1 非洲绿猴肾细胞

]资料
如果您对该产品感兴趣的话,可以 sendmsg
产品名称: CV-1 非洲绿猴肾细胞
产品型号: CCL-70 CV-1
产品厂商: 美国标准生物品收藏中心(ATCC)
产品文档: 无相关文档


简单介绍

CV-1 非洲绿猴肾细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*优培养条件!


CV-1 非洲绿猴肾细胞 的详细介绍
CV-1 非洲绿猴肾细胞
ATCC® Number: CCL-70™    Price: $289.00
Designations: CV-1
Depositors:  FC Jensen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cercopithecus aethiops
Morphology: fibroblast

Source: Organ: kidney
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: efficacy testing [92587]
transfection host (Roche FuGENE® Transfection Reagents)
viruscide testing [92582] [92579]
Virus Susceptibility: poliovirus 1; herpes simplex; Eastern equine encephalitis; Western equine encephalitis; California encephalitis; SV40
Reverse Transcript: negative
Cytogenetic Analysis: This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%. Only a few markers were found., Of these M1, a probable deleted N11, was found in all cells examined; M3 of unknown origin was in some cells; and the remaining 2 to 3 others of unknown origins were found only once., N11 was uniformly single copied, and N16 was also single copied in most cells. Both X and Y chromosomes were also detected in every cell.
Age: 141 days
Gender: male
Comments: Suitable host for transfection, especially by SV40 vectors.
The CV-1 cell line was derived from the kidney of a male ***** African green monkey by F.C. Jensen, et al. in March, 1964 for use in Rous sarcoma virus transformation studies. [22138]
Derived from the kidney of male ***** African green monkey.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
产品留言
标题
联系人
联系电话
内容
验证码
点击换一张
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

联系我们
产品搜索

细胞系,ATCC细胞,ATCC菌种,细胞系目录,ATCC,鼠伤寒沙门氏菌,atcc细胞库,人淋巴瘤细胞,人食管癌细胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌种,细胞株,标准菌株,ATCC中国,atcc上海代理,CMCC 菌株,ATCC菌株,细胞目录,质控菌株,肿瘤细胞株,乳腺癌细胞系,人肝癌细胞系

产品目录

Copyright© 2003-2020  上海复祥生物科技有限公司版权所有            电话:021-35080510
传真:021-35080520    地址:上海市虹口区赤峰路630号南雁大厦1901室    邮编: 沪ICP备10013034号  
  

沪公网安备 31010902002429号