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CRL-12103 FDC-P1 小鼠正常骨髓细胞

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产品名称: CRL-12103 FDC-P1 小鼠正常骨髓细胞
产品型号: FDC-P1
产品厂商: 美国标准生物品收藏中心(ATCC)
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CRL-12103 FDC-P1 小鼠正常骨髓细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!


CRL-12103 FDC-P1 小鼠正常骨髓细胞 的详细介绍
CRL-12103 FDC-P1 小鼠正常骨髓细胞
ATCC® Number: CRL-12103™    Price: $355.00
Designations: FDC-P1
Depositors:  University of Virginia Patent Foundation
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (mouse)
Morphology: lymphoblast

Source: Organ: bone marrow
Strain: DBA/2
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (Nucleofection technology from Lonza)
Antigen Expression: Thy-1.2 (24%)
Cytogenetic Analysis: diploid
Comments: The FDC-P1 cell line was established from long term culture of normal DBA/2 bone marrow cells in medium conditioned by WEHI-3 cells (see ATCC TIB-68).
They are dependent upton WEHI-3 conditioned medium (WEHI-3CM) for continued growth.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 65%; fetal bovine serum, 10% supplemented with 25% mouse Interleukin-3 culture supplement. (Supplement purchased from BD Biosciences, Catalog No. 354040).
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor temperature
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