产品[
CCL-97 R2C 大鼠睾丸间质细胞瘤
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产品名称:
CCL-97 R2C 大鼠睾丸间质细胞瘤
产品型号:
CCL-97
产品厂商:
美国标准生物品收藏中心（ATCC）
产品文档:
无相关文档
简单介绍
CCL-97 R2C 大鼠睾丸间质细胞瘤，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和Z优培养条件！
CCL-97 R2C 大鼠睾丸间质细胞瘤
的详细介绍
CCL-97 R2C 大鼠睾丸间质细胞瘤
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CCL-97™
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$438.00
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R2C
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G Sato
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Biosafety Level:
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1
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frozen
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Medium & Serum:
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See Propagation
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adherent
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Rattus norvegicus (rat)
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epithelial
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Strain: WFu
Organ: testis
Disease: Leydig cell tumor
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steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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transfection host (Roche FuGENE® Transfection Reagents)
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poliovirus 2
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positive
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2 months
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male
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ATCC complete growth medium: The base medium for this cell line is F-12 Nutrient Mixture (Ham). To make the complete growth medium, add the following components to the base medium:
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fetal bovine serum to a final concentration of 2.5%
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horse serum to a final concentration of 15%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
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Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Add fresh medium at least twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
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culture medium 95%; DMSO, 5%
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recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
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1100: Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022
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ATCC® Number: CCL-97™ Price: $438.00
Designations: R2C
Depositors: G Sato
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus (rat)
Morphology: epithelial
Source: Strain: WFu
Organ: testis
Disease: Leydig cell tumor
Cellular Products: steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Virus Resistance: poliovirus 2
Reverse Transcript: positive
Age: 2 months
Gender: male
Comments: Stimulation by other hormones or cAMP is not required for hormone secretion.
Propagation: ATCC complete growth medium: The base medium for this cell line is F-12 Nutrient Mixture (Ham). To make the complete growth medium, add the following components to the base medium:
•fetal bovine serum to a final concentration of 2.5%
•horse serum to a final concentration of 15%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Add fresh medium at least twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
References: 1100: Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022
