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  • 产品名称：CRL-1992 T2(174×CEM.T2) 人**母细胞

  • 产品型号：CRL-1992
  • 产品厂商：美国标准生物品收藏中心（ATCC）
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CRL-1992 T2(174×CEM.T2) 人**母细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
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CRL-1992 T2(174×CEM.T2) 人**母细胞
ATCC® Number: CRL-1992™    Price: $329.00
Designations: T2 (174 x CEM.T2)
Depositors:  P Cresswell
Biosafety Level: 2
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Antigen Expression: HLA A2; CD7 +
DNA Profile (STR): Amelogenin: X
CSF1PO: 11, 12
D13S317: 11, 12
D16S539: 10, 12
D5S818: 12, 14
D7S820: 10, 11, 15
THO1: 6, 7, 9.3
TPOX: 8, 12
vWA: 14, 17, 20
Comments: This line is a variant of the T1 cell line (ATCC CRL-1991) produced by selection the SFR1-MI.3 monoclonal antibody (against a monomorphic determinant on HLA DR).
The cells do not express HLA DR and are Class II major histocompatibility (MHC) antigen negative.
The cells synthesize, but do not express, HLA B5.
T2 appears to have lost both copies of the CEMR.3 derived chromosome 6.
Together the T1 and T2 lines are useful for studying antigen processing and T cell recognition of class I MHC antigens.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) viable cells/ml and maintain between 3 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 22647: Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841
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