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CRL-1993 HMy2.CIR 人B**母细胞
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产品名称:
CRL-1993 HMy2.CIR 人B**母细胞
产品型号:
CRL-1993
产品厂商:
美国标准生物品收藏中心(ATCC)
产品文档:
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简单介绍
CRL-1993 HMy2.CIR 人B**母细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!
CRL-1993 HMy2.CIR 人B**母细胞
的详细介绍
CRL-1993 HMy2.CIR 人B**母细胞
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CRL-1993™
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HMy2.CIR [C1R, HMy2.C1R]
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P Cresswell
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Biosafety Level:
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2 [Cells contain Eptein-Barr Viral DNA sequences ]
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frozen
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Medium & Serum:
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See Propagation
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suspension
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Homo sapiens (human)
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lymphoblast
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Cell Type: B lymphoblast;
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8
TPOX: 8
vWA: 17
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) viable cells/ml.
Interval: Maintain between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
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Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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parental cell line:ATCC CRL-1621
derivative:ATCC CRL-2369
derivative:ATCC CRL-2370
derivative:ATCC CRL-2371
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23312: Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569
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