产品[
CCL-257 NCI-H1688 人经典小细胞肺癌细胞
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产品名称:
CCL-257 NCI-H1688 人经典小细胞肺癌细胞
产品型号:
CCL-257
产品厂商:
美国标准生物品收藏中心（ATCC）
产品文档:
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简单介绍
CCL-257 NCI-H1688 人经典小细胞肺癌细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
CCL-257 NCI-H1688 人经典小细胞肺癌细胞
的详细介绍
CCL-257 NCI-H1688 人经典小细胞肺癌细胞
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CCL-257™
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$417.00
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NCI-H1688 [H1688]
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AF Gazdar, JD Minna
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Biosafety Level:
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1
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frozen
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Medium & Serum:
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial
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Organ: lung
Disease: carcinoma; classic small cell lung cancer
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Amelogenin: X
CSF1PO: 12
D13S317: 8
D16S539: 13
D5S818: 10
D7S820: 12
THO1: 6,8
TPOX: 11
vWA: 17
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50 years
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male
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
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Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, and add fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes at room temperature.
Remove the trypsin and incubate the flask at 37C for 5 to 10 minutes or until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
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Culture medium, 95%; DMSO, 5%
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
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