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  • 产品名称：CRL-1838 M-NFS-60 小鼠白血病细胞G-CSF依赖性

  • 产品型号：CRL-1838
  • 产品厂商：美国标准生物品收藏中心（ATCC）
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CRL-1838 M-NFS-60 小鼠白血病细胞G-CSF依赖性，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
详情介绍：
CRL-1838 M-NFS-60 小鼠白血病细胞G-CSF依赖性
ATCC® Number: CRL-1838™    Price: $349.00
Designations: M-NFS-60
Depositors:  JF Weaver, A Creasey
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (mouse)
Morphology: lymphoblast
Source: Disease: myelogenous leukemia
Cell Type: virus induced
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Comments: The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus.
The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF).
The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • 62 ng/ml human recombinant macrophage colony stimulating factor (M-CSF)
  • fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium at the time of subculture
Cultures can be maintained by addition or replacement of fresh medium. Subcuture every two days at 2.5 X 10 exp4 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22842: Nakoinz I, et al. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J. Immunol. 145: 860-864, 1990. PubMed: 2142710
23295: Weinstein Y, et al. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc. Natl. Acad. Sci. USA 83: 5010-5014, 1986. PubMed: 3014527
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