产品[
CCL-86 Raji 人Burkitt’s**瘤细胞
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产品名称:
CCL-86 Raji 人Burkitt’s**瘤细胞
产品型号:
CCL-86
产品厂商:
美国标准生物品收藏中心（ATCC）
产品文档:
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简单介绍
CCL-86 Raji 人Burkitt’s**瘤细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
CCL-86 Raji 人Burkitt’s**瘤细胞
的详细介绍
CCL-86 Raji 人Burkitt’s**瘤细胞
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CCL-86™
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$272.00
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Raji
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W Henle
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Biosafety Level:
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2 [CELLS CONTAIN HERPESVIRUS (EBV) ]
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frozen
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Medium & Serum:
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See Propagation
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suspension
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Homo sapiens (human)
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lymphoblast
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Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Isolation date: 1963
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transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
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The cells are partially resistant to poliovirus and vesicular stomatitis viruses.
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Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 16,19
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Karyotype 100% stable within male diploid stemline of 46. Cells with 47 chromosomes frequently contained an extra "E" group chromosome.; There is 6% polyploidy and occasional disparity in the size of the homologs of the number 1 chromosome and the number 4 chromosome.
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11 years
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male
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Black
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
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Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively the cells may be collected by centrifugation. Cultures can then be established by resuspending the cells in fresh medium at 4 X 10(5) viable cells/ml. A maximum of 3 X 10(6) viable cells/ml is obtainable.
Medium Renewal: Every 2 to 3 days
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Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derivative:ATCC CCL-214
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