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大鼠肝癌细胞系

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产品名称: 大鼠肝癌细胞系
产品型号: CRL-1600
产品厂商: 美国标准生物品收藏中心(ATCC)
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简单介绍

CRL-1600 H4-ⅡE 大鼠肝癌细胞系,原代细胞、细胞系。细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件,


大鼠肝癌细胞系 的详细介绍
CRL-1600 H4-ⅡE 大鼠肝癌细胞系
ATCC® Number: CRL-1600™    Price: $355.00
Designations: H4-II-E-C3
Depositors:  JE Becker
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus (rat)
Morphology: epithelial

Source: Organ: liver
Strain: AxC
Disease: hepatoma
Cellular Products: tyrosine aminotransferase; albumin; transferrin; prothrombin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Tumorigenic: Yes
Gender: male
Comments: Tyrosine amino transferase is inducible with glucorticoids, insulin or cAMP derivatives.
The cells are productively infected with a retrovirus.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 75%; horse serum, 20%; fetal bovine serum, 5%.
Temperature: 37.0°C
Subculturing: Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

      Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended
      Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
References: 1084: Potter VR, Morse PA Jr.. Pyrimidine metabolism in tissue culture cells derived from rat hepatomas. I. Suspension cell cultures derived from the Novikoff hepatoma. Cancer Res. 25: 499-508, 1965. PubMed: 14297488
1217: Reuber MD. A transplantable bile-secreting hepatocellular carcinoma in the rat. J. Natl. Cancer Inst. 26: 891-899, 1961. PubMed: 13740982
26102: Weinstein IB, et al. Type C virus from cell cultures of chemically induced rat hepatomas. Science 178: 1098-1100, 1972. PubMed: 4343844
33030: Klemm DJ, et al. Adenovirus E1A proteins regulate phosphoenolpyruvate carboxykinase gene transcription through multiple mechanisms. J. Biol. Chem. 271: 8082-8088, 1996. PubMed: 8626493
58076: Peraino C, et al. Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233
 
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