|
|
RWPE-2
|
|
|
Michigan State University, National Cancer Institute
|
|
Biosafety Level:
|
2 [Cells contain Human Papilloma viral (HPV) sequences ]
|
|
|
frozen
|
|
Medium & Serum:
|
See Propagation
|
|
|
adherent
|
|
|
Homo sapiens (human)
|
|
|
CRL-11610 RWPE-2 人前列腺正常细胞epithelial
|
|
|
Organ: prostate
Disease: normal
Cell Type: epithelial
|
|
|
cytokeratin 18 [46793]
cytokeratin 8 [46793]
|
|
|
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
|
|
|
|
|
androgen receptor, expressed ( [46793] upregulated upon exposure to androgen)
|
|
|
Yes
|
|
|
kallikrein 3, KLK3 (prostate specific antigen, PSA); Homo sapiens, expressed (upon exposure to androgen) ( [46793] upon exposure to androgen)
|
|
|
Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,14
D16S539: 9,11
D5S818: 12,15
D7S820: 10,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14,18
|
|
|
At passage 14, a majority of the cells were in the near diploid range (48-54), with the main population being 51, XY. [46795]
|
|
|
AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 1-2
Me-2, 0
PGM1, 2
PGM3, 1
|
|
|
CRL-11610 RWPE-2 人前列腺正常细胞male
|
|
|
Caucasian, White
|
|
|
ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
-
0.05 mg/ml BPE - provided with the K-SFM kit
-
5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
|
|
|
CRL-11610 RWPE-2 人前列腺正常细胞Protocol:
-
Remove and discard culture medium.
-
Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS).
-
Add 2.0 to 3.0 ml (to a T-25 flask) or 3.0 to 4.0 ml (to a T-75 flask) of 0.05% Trypsin - 0.53mM EDTA solution, diluted 1:1 with D-PBS, and place flask in a 37C incubator for 5 to 8 minutes. Observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.
-
Add 6.0 to 8.0 ml of 0.1% Soybean Trypsin Inhibitor (or 2% fetal bovine serum in D-PBS), as appropriate, and aspirate cells by gently pipetting.
-
Transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 7 minutes.
-
Discard supernatant and resuspend cells in fresh serum-free growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 2 X 10(4) to 4 X 10(4) viable cells/sq. cm is recommended.
-
Incubate cultures at 37C. We recommend that you maintain cultures at a cell concentration between 4 X 10(4) and 8 X 10(4) cells/sq. cm.
Cells grown under serum-free or reduced serum conditions may not attach strongly during the 24 hours after subculture and should be disturbed as little as possible during that period.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 days
|
|
|
Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
|
|
|
parental cell line:ATCC CRL-11609
purified DNA:ATCC CRL-11610D
derived from same individual:ATCC CRL-2849
derived from same individual:ATCC CRL-2850
derived from same individual:ATCC CRL-2852
derived from same individual:ATCC CRL-2851
derivative:ATCC CRL-2853
derived from same individual:ATCC CRL-2854
|
|
|
46792: Webber MM, Rhim JS. Immortalized and malignant human prostatic cell lines. US Patent 5,824,488 dated Oct 20 1998
46793: Bello D, et al. Androgen responsive ***** human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18: 1215-1223, 1997. PubMed: 9214605
46795: Webber MM, et al. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 18: 1225-1231, 1997. PubMed: 9214606
53180: Webber MM, et al. Immortalized and tumorigenic ***** human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636
53181: Webber MM, et al. Immortalized and tumorigenic ***** human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337
53182: Webber MM, et al. Immortalized and tumorigenic ***** human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152
53183: Kremer R, et al. ras Activation of human prostate epithelial cells induces overexpression of parathyroid hormone-related peptide. Clin. Cancer Res. 3: 855-859, 1997. PubMed: 9815759
53192: Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497
|
CRL-11610 RWPE-2 人前列腺正常细胞
|
