产品[
CRL-1999 T/G HA-VSMC
]资料
如果您对该产品感兴趣的话,可以
产品名称:
CRL-1999 T/G HA-VSMC
产品型号:
CRL-1999 人血管平滑肌
产品厂商:
美国标准生物品收藏中心（ATCC）
产品文档:
无相关文档
简单介绍
CRL-1999 T/G HA-VSMC 人血管平滑肌，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
CRL-1999 T/G HA-VSMC
的详细介绍
CRL-1999 T/G HA-VSMC 人血管平滑肌
|
|
CRL-1999™
|
|
$264.00
|
|
|
T/G HA-VSMC
|
|
|
RR Gifford
|
|
Biosafety Level:
|
1
|
|
|
frozen
|
|
Medium & Serum:
|
See Propagation
|
|
|
adherent
|
|
|
Homo sapiens (human)
|
|
|
fibroblast
|
|
|
Organ: aorta
Tissue: smooth muscle
Disease: normal
|
|
|
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
|
|
|
|
|
transfection host (technology from amaxa)
|
|
|
N
|
|
|
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 12
D5S818: 12,13
D7S820: 9,10
THO1: 7,8
TPOX: 8
vWA: 16
|
|
|
This is a normal human cell line with a 46,XX karyotype. The modal chromosome number was 46, occurring in 90% of cells. The rate of polyploid cells was 0.8%. No structurally altered chromosomes were detected in 15 metaphases karyotyped. Both X chromosomes appeared normal.
|
|
|
11 months
|
|
|
female
|
|
|
Caucasian
|
|
|
N
|
|
|
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement (ECGS); fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM, TES to a final concentration of 10 mM.
Temperature: 37.0°C
|
|
|
Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, and incubate at 37C for 5 minutes. Add an equal volume of fresh medium, disperse the cells and centrifuge to pellet the cells. Resuspend the cells in fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
|
|
|
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
|
|
|
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
|
|
