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SVEC4-10
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柯·奥康奈尔
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生物**等级:
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2 [细胞含有多瘤病毒DNA序列]
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冷冻的
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中和血清:
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见传播
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依附的
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小家鼠(小鼠)
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上皮的
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器官:腋窝**结
株: C3H / HeJ
组织:血管上皮
细胞类型:膀胱SV40转化
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除了上面提到的MTA之外,此ATCC材料的转移可能还需要其他ATCC和/或法规许可。购买ATCC材料的任何人*终都有责任获得许可。请注意此处以了解有关运送到您所在地的特定要求的信息。
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低密度脂蛋白(LDL)的高亲和力受体
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是
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H-2 K;VIII因子相关抗原;VCAM
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男
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ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
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Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
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培养基95%;DMSO,5%
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24至30小时
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推荐的培养基(不含ATCC培养基中所述的其他补充剂或血清):ATCC 30-2002
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22840:O'Connell KA,EdindinM。被猿猴病毒40永生的小鼠**样胚胎细胞系结合**细胞并保留正常胚胎细胞的功能特性。J.**。144:521-525,1990。考研:2153170
23172 :O'Connell KA,Rudmann AA。来自鼠卡波西氏肉瘤样肿瘤的梭形和上皮样细胞是胚胎来源的。J.投资。Dermatol。100:742-745,1993。考研:8496612
23187:O “康奈尔ķ等。SV40Ť抗原转化的内皮细胞在裸鼠中引起卡波西氏肉瘤样肿瘤。上午。J.Pathol。139:743-749,1991.PubMed:1928299
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