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CCL-120.1 CCRF HSB-2 人急性**母细胞型白血病细胞

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产品名称: CCL-120.1 CCRF HSB-2 人急性**母细胞型白血病细胞
产品型号: CCL-120.1
产品厂商: 美国标准生物品收藏中心(ATCC)
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CCL-120.1 CCRF HSB-2 人急性**母细胞型白血病细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!


CCL-120.1 CCRF HSB-2 人急性**母细胞型白血病细胞 的详细介绍
CCL-120.1 CCRF HSB-2 人急性**母细胞型白血病细胞
ATCC® Number: CCL-120.1™    Price: $338.00
Designations: CCRF-HSB-2 [CCRF HSB-2, HSB-2]
Depositors:  GE Foley
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast

Source: Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: T lymphoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Tumorigenic: Yes
Antigen Expression: HLA A1, A2, B12, B17, Cw2; CD5 (78%), CD7 (96%)
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18,19
Isoenzymes: G6PD, B
Age: 11.5 years
Gender: male
Ethnicity: Caucasian
Comments: Derived from the same buffy coat preparation as CCL-120 (CCRF-SB) by serially transplanting into newborn syrian hamsters.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 - 5 X 10 exp5 viable cells/ml.
A maximum of 2 - 3 X 10 exp6 viable cells/ml is obtainable.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
derived from same individual:ATCC CCL-120
References: 24413: . M.D. Anderson Hospital and Tumor Inst. Monograph. 21: ; 1967.
25969: Adams RA, et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121-1125, 1968. PubMed: 4872716
26035: . . Proc. Am. Assoc. Cancer Res. 8: 1, 1967.
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