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CRL-1972 M2-10B4 小鼠骨髓纤维原细胞
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产品名称:
CRL-1972 M2-10B4 小鼠骨髓纤维原细胞
产品型号:
CRL-1972
产品厂商:
美国标准生物品收藏中心（ATCC）
产品文档:
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简单介绍
CRL-1972 M2-10B4 小鼠骨髓纤维原细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理范，提供的细胞株背景清楚，提供参考文献和Z优培养条件！
CRL-1972 M2-10B4 小鼠骨髓纤维原细胞
的详细介绍
CRL-1972 M2-10B4 小鼠骨髓纤维原细胞
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CRL-1972™
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$349.00
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M2-10B4
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CJ Eaves
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Biosafety Level:
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1
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frozen
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Medium & Serum:
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See Propagation
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adherent
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Mus musculus (mouse)
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fibroblast
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Organ: bone marrow
Tissue: stroma
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laminin; collagen IV
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
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Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
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Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
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22620: Sutherland HJ, et al. Differential regulation of primitive human hematopoietic cells in long- term cultures maintained on genetically engineered murine stromal cells. Blood 78: 666-672, 1991. PubMed: 1713512
22769: Lemoine FM, et al. Partial characterization of a novel stromal cell-derived pre-B-cell growth factor active on normal and immortalized pre-B cells. Exp. Hematol. 16: 718-726, 1988. PubMed: 3261251
23070: Lemoine FM, et al. Autocrine production of pre-B-cell stimulating activity by a variety of transformed murine pre-B-cell lines. Cancer Res. 48: 6438-6443, 1988. PubMed: 3263188
23353: Lemoine FM, et al. Transformation-associated alterations in interactions between pre-B cells and fibronectin. Blood 76: 2311-2320, 1990. PubMed: 2147863
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