产品[
CRL-1872 A375.S2 人黑色素瘤细胞
]资料
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产品名称:
CRL-1872 A375.S2 人黑色素瘤细胞
产品型号:
CRL-1872
产品厂商:
美国标准生物品收藏中心（ATCC）
产品文档:
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简单介绍
CRL-1872 A375.S2 人黑色素瘤细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！
CRL-1872 A375.S2 人黑色素瘤细胞
的详细介绍
CRL-1872 A375.S2 人黑色素瘤细胞
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CRL-1872™ |
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$417.00 |
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A375.S2 |
| Biosafety Level: |
1 |
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frozen |
| Medium & Serum: |
See Propagation |
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adherent |
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Homo sapiens (human) |
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Organ: skin
Disease: malignant melanoma |
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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Yes |
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Amelogenin: X
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17 |
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54 years |
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female |
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
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Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution and let the culture sit at room temperature (or 37C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
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culture medium 95%; DMSO, 5% |
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-1619 |
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1773: Onozaki K, et al. Human interleukin-1 is a cytocidal factor for several tumor cell lines. J. Immunol. 135: 3962-3968, 1985. PubMed: 2415593
1774: Lachman LB, et al. Natural and recombinant human interleukin-1beta is cytotoxic for human melanoma cells. J. Immunol. 136: 3098-3102, 1986. PubMed: 3485680
1775: Nakai S, et al. A simple, sensitive bioassay for the detection of interleukin-1 using human melanoma A375 cell line. Biochem. Biophys. Res. Commun. 154: 1189-1196, 1988. PubMed: 3261584
23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 |
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